Analysis of Marine Samples in Search of Bioactive Compounds by Teresa Rocha-Santos, Armando C. Duarte

By Teresa Rocha-Santos, Armando C. Duarte

Seas and oceans supply a variety of temperature, strain, mild and chemical conditions thus permitting a large range of marine organisms from shallow coastal waters to the deep ocean. those assets can be utilized to procure new items and advance companies, and in flip support to provide solutions to the demanding situations that have an effect on our planet, together with providing a sustainable provide of meals and effort, new business fabrics and procedures, new bioactive compounds, and new well-being remedies. Marine compounds were pointed out as having antibacterial, anticoagulant, antifungal, antimalarial, antiprotozoal, antituberculosis, and antiviral actions. the most important resources of those bioactive compounds are marine sponges, coelenterates, and microorganisms, via algae, echinoderms, tunicates, molluscs, and bryozoans.

The discovery of bioactive compounds from marine samples is a sizzling subject contemplating the present need for sustainable use of marine assets. This e-book is a accomplished evaluate of the analytical techniques employed within the discovery and characterization of bioactive compounds remoted from (all attainable) marine samples and provides destiny views of analytical methodologies. This evaluate comprises an overview of the sampling and education of extracts, the separation and isolation of bioactive compounds, their structural characterization and the applying of bioassays within the discovery of bioactive compounds.

  • Comprehensive insurance of analytical suggestions and applications
  • Clear diagrams to competently aid vital topics
  • Real examples of functions of analytical recommendations within the look for new bioactive compounds

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2). Another very important aspect is the collection method. Before entering into detail, it is important to remember that biodiversity conservation should always be kept in mind, mitigating the impacts as much as possible. Collection of organisms must be restricted to minimum amounts and care with rare or endangered species must be taken into account, together with care in preserving the surrounding individuals and communities. The first step that must be assured when collecting specimens from wild populations during the prospection process is that the organisms are accurately identified (at least to the nearest taxonomic level), that the location of collection is accurate (preferably GPS coordinates), and also that they are in perfect condition to be fully evaluated, either by chemical screening or through bioassays [2,45].

1); that is, constituents that exhibit phytochemical properties, as studied by Marimuthu et al. [12]. Halim et al. [57] have extracted lipids from microalgae Chlorococcum sp. through supercritical carbon dioxide (a green extraction methodology, discussed in Chapter 4) and Soxhlet extraction with hexane, in order to compare their extract yields. Halim et al. 058 g of lipid extract per g of dried microalgae) with less efficiency recorded with the Soxhlet procedure. Spiric et al. [58] also compared the extraction of marine bioactive compounds (fatty acids and cholesterol) in carp fish muscles obtained with a green extraction methodology; that is, the pressurized liquid extraction (also discussed in Chapter 4) and the classical methodology of Soxhlet extraction.

6]. Nevertheless, the marine environment has a wide variety of other organisms such as bacteria, algae, and invertebrates such as sponges, corals, and tunicates, which could be used as sources of bioactive compounds with pharmaceutical and therapeutical interest. This chapter presents state of the art strategies based on classical methodologies for the isolation of the bioactive compounds found in the marine environment organisms, including the preparation of extracts and fractions. 2). 2, there is a plethora of marine organisms that could be useful as a source of bioactive compounds.

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