A Manual of Chemical & Biological Methods for Seawater by Timothy Richard Parsons

By Timothy Richard Parsons

An advent to the quantitative research of seawater, describing intimately organic and chemical innovations, that are thought of to be among these in most cases utilized by organic oceanographers. The handbook offers whole directions for the addition of reagents and calculation of effects in regards fabric for every approach in order that the unique texts should be consulted if helpful. typically, the recommendations require no less than past specialist education and strategies desiring very pricey apparatus were shunned

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Nat. Acad. , USA, 72: 619, 1975), Josefsson et al. (Anal. Chim. Acta, 89: 21,1977), Dawson and Pritchard (Mar. , 6: 27, 1978) and Lindroth and Mopper (Anal. , 51: 1667, 1979). Free amino acids, peptides, and proteins in seawater are present in trace amounts. The desalting and concentration of large volumes of seawater samples have usually been needed in order to analyze for amino acids. The techniques depend mainly on the sensitivity of the determinations using such methods as spectrophotometry and thin layer and paper chromatography.

7N HC1. F. Experimental procedure 1. Sample solutions should be brought to a temperature between about 18° and 20°C. 0 ml portion (in triplicate) of the water sample into 10-ml 50 Soluble Organic Material capacity screw-capped test tubes with teflon liners. 05 ml of potassium borohydride solution into each test tube. Allow the solution to stand for 4 hr to complete reduction at 18°C in the dark. 2. 7N HC1. Remove the cap of the test tube and leave for 10 min at room temperature to allow hydrogen gas to evolve.

2. Add the reducing reagent rapidly (it can be dispensed from a wash bottle) to make 50 ml and mix immediately. 3. Allow the solution to stand for 2-3 hr (Note a) to complete the reduction. Measure the extinction at 810 nm using a 1-cm cell for concentrations > 15 /xg-at/1 and a 10-cm cell for concentrations of < 15 /xg-at/1. 4. Correct the measured extinction for the blank ( 1 - or 10-cm cell length) and calculate reactive silicate as: /xg-at Si/1 = corrected extinction x F where F\s the factor for 1- or 10-cm cell length as defined below.

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